Journal article
Generation of immortalized human endometrial stromal cell lines with different endometriosis risk genotypes
SJ Holdsworth-Carson, EM Colgrave, JF Donoghue, JN Fung, ML Churchill, S Mortlock, P Paiva, M Healey, GW Montgomery, JE Girling, PAW Rogers
Molecular Human Reproduction | OXFORD UNIV PRESS | Published : 2019
Abstract
Endometriotic lesions are composed in part of endometrial-like stromal cells, however, there is a shortage of immortalized human endometrial stromal cultures available for research. As genetic factors play a role in endometriosis risk, it is important that genotype is also incorporated into analysis of pathological mechanisms. Human telomerase reverse transcriptase (hTERT) immortalization (using Lenti-hTERT-green fluorescent protein virus) took place following genotype selection; 13 patients homozygous for either the risk or non-risk â € other' allele for one or more important endometriosis risk single nucleotide polymorphism on chromosome 1p36.12 (rs3820282, rs56318008, rs55938609, rs120373..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
All authors declare that they have no competing financial interests. Research reported in this publication was supported in part by National Health and Medical Research Council (NHMRC) project grants GNT1012245 and GNT1105321 (P.A.W.R., G.W.M., J.E.G., S.J.H.-C.). G.W.M. is supported by the NHMRC (GNT1078399 and GNT1026033, GNT1049472, and GNT1050208). P.A.W.R. was supported by a Bayer Group Grants4Targets grant. S.J.H.-C. was supported by the J.N. Peters Bequest Fellowship, University of Melbourne (2016).